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For 5-Amino-1MQ, this meant enzymatic assays confirming substrate-competitive inhibition of NNMT, selectivity screens against related methyltransferases to rule out promiscuous activity, and cell-permeability testing to confirm the molecule crosses membranes to its intracellular target
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It is important to note that these side effects are relatively uncommon and, when they do occur, are usually mild and temporary
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