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To investigate whether PM phospholipids scrambling occurs during the initiation of NET formation, we employed the classical NOX2-dependent NET activator, phorbol 12-myristate 13-acetate (PMA) in serum-free RPMI 1640 medium supplemented with 10 mM HEPES, to stimulate polymorphonuclear neutrophils (PMNs) purified from mouse bone marrow
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