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Cell lysates were collected on ice at the indicated time points with RIPA buffer (50 mM Tris–HCl pH 8, 150 mM NaCl, 1% NP40, 0.1%SDS and 0.5% Na Deoxycholate) supplemented with complete EDTA-free protease inhibitors and PhosSTOP phosphatase inhibitor cocktail for western blot analysis
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