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Serial monitoring of clonal evolution—such as tracking trunk mutations (reflecting TMB) and resistance-associated variants (e.g., RAS/BRAF) in circulating tumor DNA (ctDNA)—further enables dynamic assessment of therapeutic vulnerability, as demonstrated in metastatic colorectal cancer (Vidal et al., 2023)
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[DOI] [PMC free article] [PubMed] [Google Scholar] 211.Seven D., Dalan A.B., Bayrak Ö.F
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