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the culture medium was exchanged by an extracellular solution containing (in mM) 140 NaCl, 2.5 KG, 1 MgCl 2 , 3 CaCI 2 , 25 glucose, and 5 HEPES
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Neurotrophic agents such as nerve growth factor and brain-derived neurotrophic factor have received research attention concerning their potential to treat AD but have not progressed to clinical trials due to their reasonably large size, inability to penetrate the blood-brain barrier (BBB), and the high cost of synthesis
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