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Activation of the Ah receptor signal transduction pathway by bilirubin and biliverdin

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Membranes were blocked for 1 h at room temperature with 5% w/v blotting buffer (Biorad) in Tris-buffered saline with 0.1% v/v Tween-20 (TBS-T), then incubated with primary antibodies in 5% w/v blotting buffer in TBS-T overnight at 4°C, with shaking