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Diagnostic methods Glutathione and sulphydryl compunds in erythrocytes can be measured using 5,5'-dithiobis (2-nitrobenzoic acid), while glutathione in fibroblasts can be measured with the 5,5'-dithiobis (2-nitrobenzoic acid) glutathione recycling assay [16], or using high performance liquid chromatography (HPLC) [17]

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This is evident where depletion of NAD + can prevent immune cell proliferation and differentiation, reduce viability, and blunt distinct immune cell processes such as phagocytosis, in parallel with NAD + -boosting blunting inflammation whilst at the same time increasing CTL activity