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Die affiniteit is een eigenschap van de aminozuurvolgorde zelf en is de reden dat juist dit fragment wordt gesynthetiseerd in plaats van het volledige eiwit
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Early approaches employed random amine coupling of full-length exendin-4 using 4-Maleimidobutyric acid N-hydroxysuccinimide ester (GMBS) to tether the peptide to Cy5.5-labelled iron oxide cores, forming the MN-Ex5/10-Cy5.5 probe
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