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Approximately 100 ng of total RNA was reverse transcribed, and the first strand of cDNA was obtained by reverse transcription by using GeneRuler DNA Ladder Mix and Maxima Reverse Transcriptase (Thermo Fisher Scientific, Massachusetts, USA) as the manufacturer’s instructions
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The spectroscopic properties of the double and triple mutants shed light on the effect of the single mutant combinations on the photobleaching and acidification behavior (Fig
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