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Silanization of coverslips: 18 × 18 mm glass coverslips (Thermo Fischer Scientific) were placed in a coverslip rack and carefully put into an acetone solution containing 1% (3-aminopropyl) triethoxysilane (APTES) and 1% allyltrimethoxysilane (ATMS) (v/v) and coated overnight 26
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Actions through IGFBP-2 MiR-130b MiR-130b-5p and miR-130b-3p, the two strands of the miR-130b duplex, appear to have independent actions on IGF-axis proteins in non-cancer models, which may be relevant in some cancers
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