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The staining results were observed and imaged using an Axio Observer 7 microscope (Carl Zeiss Meditec AG, Jena, Germany)
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In contrast to purines that are synthesized as ribonucleotides rather than as nucleobases, pyrimidines are synthesized first as nucleobases and then conjugated to phosphoribosyl pyrophosphate (PRPP) to yield the corresponding ribonucleotide
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