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IGF-1 treatment promoted neuronal maturation in a spatiotemporal manner To measure whether systemic treatment with IGF-1 influences the neuronal cell population in the developing pig brain, the number of mature neurons (NeuN + cells) and total area of NeuN-IR were quantified in the parietal and entorhinal cortex, subiculum, and different subregions of the dorsal and ventral hippocampus of control and IGF-1-treated pigs at P5 and P9 (Fig

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