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Briefly, 400 μm slices were cultured on (Milipore, Billerica, Mass.) for 3 days after which they were biolistically transfected with tomato fluorescent protein (TFP) using a Helios Gen Gun (BioRad, Hercules, Calif.), according to the manufacturer's protocol, to visualize dendritic arbors

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The fact that IGF1-SDS values increased significantly in our cohort, whereas IGFBP3-SDS values showed a trend toward increase, is consistent with the knowledge that IGF1 usually responds more markedly to stimuli than IGFBP3 [35]