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The CYP4A22 protein is highly homologous with CYP4A11 and has been shown to exhibit lauric acid ω-hydroxylase activity

type_Système immunitaire

Quantity:1 PCS

RNA was reverse transcribed into cDNA (50 ng RNA per 20 μ l RT reaction) using High Capacity RNA to cDNA kits (Life Technologies) according to the manufacturer’s instructions, and the expression of GSTP1 (Taqman probe ID Hs00168310_m1) and the loading control 18S ribosomal RNA (Hs03003631_g1) assessed by qRT–PCR analysis, as previously described (Smith et al, 2012), where 20 μ l individual reaction mixes (per well) contained 10 μ l Taqman Universal PCR Master Mix (Life Technologies), 1 μ l gene-specific Taqman probe, 1 μ l cDNA and 8 μ l sterile water