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To determine the half maximal inhibitory concentration (IC 50 ), 10 -9 to 10 -4 M concentration of compounds were used
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The combined p value for the trend of increased ferroptosis sensitivity with dedifferentiation across both the CTRP results and the validation results is based on correlation (p=10 −12 ) with similar results with Jonckheere-Terpstra trend test assessing ordered significance across the 4 differentiation stages (p=10 −13 )
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