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While Bd GUS does not possess a loop at the active site, as predicted by sequence analysis, it does contain a loop insert in the CBM that enters the active site and is not present in Bu GUS (Figs 3b and S4)
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Furthermore, in our recent study, we performed untargeted metabolomics and miRNAseq on mild and sever AMN and age and sex-matched healthy human plasma and observed a significant clinical correlation for 7-alpha-hydroxy-3-oxo-4-cholestenoate (7-HOCA), dehydroepiandrosterone sulfate (DHEA-S), hypoxanthine, and miRNA-432–5p [94]
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