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10.1038/cdd.2015.158
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The reaction was terminated by the addition of 200 µl/well of 6M Guanidine HCl followed by three washes of 300 µl/well with PBS, 2 M NaCl, 50 mM MgSO 4 , 0.05% TWEEN 20 detergent (Buffer B)
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